Journal: PLOS ONE
Article Title: Adipose tissue-derived exosomes alleviate particulate matter-induced inflammatory response and skin barrier damage in atopic dermatitis-like triple-cell model
doi: 10.1371/journal.pone.0292050
Figure Lengend Snippet: Fluorescence microscopy images of the intracellular uptake of ASC-exosomes (green) in the cells (plasma membrane stained red) after treatment for 6, 24, and 48 hours. Nuclei are labeled with Hoechst 33258 (blue). (A) HaCaT cells (Human Keratinocyte); (B) HDF cells (Human Primary Dermal Fibroblasts); (C) HDF + HMC-1 (Human mast cell); (D) Triple-cell culture model.
Article Snippet: The human mast cell line HMC-1 was obtained from Merck Millipore (Temecula, CA, USA).
Techniques: Fluorescence, Microscopy, Membrane, Staining, Labeling, Cell Culture